Anti-Human ACSL3 Polyclonal Antibody (HF536014)

Name: Anti-Human ACSL3 Polyclonal Antibody
Brand: abinScience
SKU: HF536014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF536014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ACSL3 (Lys581-Lys720).

Target

LACS 3, LACS3, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 3, ACS3, Long-chain-fatty-acid--CoA ligase 3, FACL3, ACSL3

Purification

Purified by antigen affinity column.

Accession

O95573

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Fatty acid CoA ligase Acsl3 is a ~80 kDa protein. Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates.

1. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490

2. Yao, H. et al. (2008) The Journal of biological chemistry 283, 849-54. PMID: 18003621

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human ACSL3 polyclonal antibody at 1ug/mL on recombinant Human ACSL3 (Catalog No. HF536012).

References

Datasheet

Anti-Human ACSL3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ACSL3 Polyclonal Antibody (HF536014)

Overview

Images

References

Datasheet

SDS

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