Anti-Human VPS4B Polyclonal Antibody (HF794014)

Name: Anti-Human VPS4B Polyclonal Antibody
Brand: abinScience
SKU: HF794014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HF794014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human VPS4B (Gly138-Gly444).

Target

SKD1, VPS4B, Suppressor of K(+) transport growth defect 1, Cell migration-inducing gene 1 protein, Protein SKD1, Vacuolar protein sorting-associated protein 4B, VPS42

Purification

Purified by antigen affinity column.

Accession

O75351

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Vacuolar protein sorting-associated protein 4B (VPS4B) is a ~49 kDa protein. Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

1. Lata, S. et al. (2008) Science (New York, N.Y.) 321, 1354-7. PMID: 18687924

2. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human VPS4B polyclonal antibody at 1ug/mL on recombinant Human VPS4B (Catalog No. HF794012).

References

Datasheet

Anti-Human VPS4B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human VPS4B Polyclonal Antibody (HF794014)

Overview

Images

References

Datasheet

SDS

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