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Anti-Human ATG2A Polyclonal Antibody (HS754014)

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Overview
Catalog No.HS754014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ATG2A (Arg233-Gly341).
Target Autophagy-related protein 2 homolog A, ATG2A, KIAA0404
Purification Purified by antigen affinity column.
Accession Q2TAZ0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Autophagy-related protein 2 homolog A (ATG2A) is a ~212 kDa protein. Lipid transfer protein involved in autophagosome assembly. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion. Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Also regulates lipid droplets morphology and distribution within the cell.

1. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066
2. Valverde, DP. et al. (2019) The Journal of cell biology 218, 1787-1798. PMID: 30952800
3. Maeda, S. et al. (2019) eLife 8. PMID: 31271352
4. Velikkakath, AK. et al. (2012) Molecular biology of the cell 23, 896-909. PMID: 22219374
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Anti-Human ATG2A Polyclonal Antibody

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