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Anti-Human GNB5 Polyclonal Antibody (HT096014)

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Overview
Catalog No.HT096014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human GNB5 (Met95-Ala395).
Target Guanine nucleotide-binding protein subunit beta-5, GNB5, Gbeta5, Transducin beta chain 5
Purification Purified by antigen affinity column.
Accession O14775
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Guanine nucleotide-binding protein subunit beta-5 (GNB5) is a ~43 kDa protein. Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, such as RGS7 and RGS9, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation. Increases RGS7 GTPase-activating protein (GAP) activity, thereby regulating mood and cognition. Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors. May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate.

1. Shamseldin, HE. et al. (2016) Genome biology 17, 195. PMID: 27677260
Note For research use only.
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Formula
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Anti-Human GNB5 Polyclonal Antibody

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