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Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

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Overview
Catalog No.HX067014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human UBE2D3 (Met1-Met147).
Target Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3
Purification Purified by antigen affinity column.
Accession P61077
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin.

1. Rajendra, R. et al. (2004) The Journal of biological chemistry 279, 36440-4. PMID: 15247280
2. Huang, J. et al. (2004) The Journal of biological chemistry 279, 54110-6. PMID: 15496420
3. Kubori, T. et al. (2008) Molecular microbiology 67, 1307-19. PMID: 18284575
4. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
5. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592
6. Peng, H. et al. (2017) Cell research 27, 657-674. PMID: 28322253
7. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421
8. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681
Note For research use only.
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Formula
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Anti-Human UBE2D3 Polyclonal Antibody

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