Anti-Human ACSS2 Polyclonal Antibody (HP274014)

Name: Anti-Human ACSS2 Polyclonal Antibody
Brand: abinScience
SKU: HP274014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP274014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ACSS2 (Asp346-Gln701).

Target

ACS, AceCS1, ACSS2, Acyl-CoA synthetase short-chain family member 2, Propionate--CoA ligase, Acyl-activating enzyme, Acetate--CoA ligase, ACAS2, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1, AceCS, Acetyl-coenzyme A synthetase, cytoplasmic

Purification

Purified by antigen affinity column.

Accession

Q9NR19

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Acetyl-coenzyme A synthetase, cytoplasmic (ACSS2) is a ~78 kDa protein. Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity. Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis. Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription.

1. Luong, A. et al. (2000) The Journal of biological chemistry 275, 26458-66. PMID: 10843999

2. Yoshimura, Y. et al. (2017) Journal of biochemistry 161, 279-289. PMID: 28003429

3. Li, X. et al. (2017) Molecular cell 66, 684-697.e9. PMID: 28552616

Note

For research use only.

Images

References

Datasheet

Anti-Human ACSS2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ACSS2 Polyclonal Antibody (HP274014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us