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Anti-Human RPS7 Polyclonal Antibody (HX848014)

Anti-Human RPS7 Polyclonal Antibody
Anti-Human RPS7 Polyclonal Antibody
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Overview
Catalog No.HX848014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RPS7 (Lys15-Leu194).
Target 40S ribosomal protein S7, RPS7, Small ribosomal subunit protein eS7
Purification Purified by antigen affinity column.
Accession P62081
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Small ribosomal subunit protein eS7 (RPS7) is a ~22 kDa protein. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for rRNA maturation. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399
2. Gazda, HT. et al. (2008) American journal of human genetics 83, 769-80. PMID: 19061985
3. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797
Note For research use only.
Images
  • Anti-Human RPS7 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RPS7 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: PC-12 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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