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Anti-Human FDX1 Polyclonal Antibody (HY268014)

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Overview
Catalog No.HY268014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human FDX1 (Asp65-Ser184).
Target Adrenodoxin, mitochondrial, Adrenal ferredoxin, Hepatoredoxin, FDX1, Ferredoxin-1, ADX
Purification Purified by antigen affinity column.
Accession P10109
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adrenodoxin, mitochondrial (FDX1) is a ~19 kDa protein. Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons.

1. Sheftel, AD. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 11775-80. PMID: 20547883
2. Strushkevich, N. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10139-43. PMID: 21636783
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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