Anti-Human MRPL58 Polyclonal Antibody (HC456014)

Name: Anti-Human MRPL58 Polyclonal Antibody
Brand: abinScience
SKU: HC456014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
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References
Datasheet

Overview

Catalog No.

HC456014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MRPL58 (Asp71-Asp206).

Target

DS1, MRPL58, Immature colon carcinoma transcript 1 protein, Digestion substraction 1, Mitochondrial large ribosomal subunit protein mL62, DS-1, ICT1, MRP-L58, Peptidyl-tRNA hydrolase ICT1, mitochondrial, 39S ribosomal protein L58, mitochondrial

Purification

Purified by antigen affinity column.

Accession

Q14197

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Large ribosomal subunit protein mL62 (MRPL58) is a ~23 kDa protein. Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. Involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.

1. Richter, R. et al. (2010) The EMBO journal 29, 1116-25. PMID: 20186120

2. Kummer, E. et al. (2021) Molecular cell 81, 2566-2582.e6. PMID: 33878294

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MRPL58 polyclonal antibody at 1ug/mL on recombinant Human MRPL58 (Catalog No. HC456012).

References

Datasheet

Anti-Human MRPL58 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MRPL58 Polyclonal Antibody (HC456014)

Overview

Images

References

Datasheet

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