Anti-Human EIF4A3/DDX48 Polyclonal Antibody (HW559014)

Name: Anti-Human EIF4A3/DDX48 Polyclonal Antibody
Brand: abinScience
SKU: HW559014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HW559014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human EIF4A3/DDX48 (Phe40-Ile411).

Target

ATP-dependent RNA helicase eIF4A-3, eIF-4A-III, Eukaryotic translation initiation factor 4A isoform 3, Eukaryotic initiation factor 4A-like NUK-34, Nuclear matrix protein 265, hNMP 265, KIAA0111, DDX48, NMP 265, eIF4A-III, Eukaryotic initiation factor 4A-III, ATP-dependent RNA helicase DDX48, EIF4A3, DEAD box protein 48

Purification

Purified by antigen affinity column.

Accession

P38919

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eukaryotic initiation factor 4A-III (EIF4A3/DDX48) is a ~46 kDa protein. ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD).

1. Ballut, L. et al. (2005) Nature structural & molecular biology 12, 861-9. PMID: 16170325

2. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638

3. Barbosa, I. et al. (2012) Nature structural & molecular biology 19, 983-90. PMID: 22961380

4. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346

5. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770

6. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 29301961

7. Gehring, NH. et al. (2005) Molecular cell 20, 65-75. PMID: 16209946

8. Tange, TØ. et al. (2005) RNA (New York, N.Y.) 11, 1869-83. PMID: 16314458

9. Bono, F. et al. (2006) Cell 126, 713-25. PMID: 16923391

Note

For research use only.

Images

References

Datasheet

Anti-Human EIF4A3/DDX48 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human EIF4A3/DDX48 Polyclonal Antibody (HW559014)

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