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Anti-DLAT Polyclonal Antibody (HY587014)

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Overview
Catalog No.HY587014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human DLAT (Gln374-Trp631).
Target DLTA, M2 antigen complex 70 kDa subunit, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Pyruvate dehydrogenase complex component E2, PDC-E2, DLAT, PBC, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, PDCE2
Purification Purified by antigen affinity column.
Accession P10515
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only.
Images
  • Western blot

    Western blot analysis was performed using anti-DLAT polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: Lncap cell lysate
    Lane 4: K562 cell lysate
    Lane 5: HCT116 cell lysate
    Lane 6: NIH3T3 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse liver lysate
    Lane 9: Mouse kidney lysate
    Lane 10: Rat brain lysate
    Lane11: Rat liver lysate
    Lane12: Rat kidney lysate

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