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Anti-XPNPEP3 Polyclonal Antibody (HV814014)

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Overview
Catalog No.HV814014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human XPNPEP3 (Glu253-Gln482).
Target Xaa-Pro aminopeptidase 3, APP3, XPNPEP3, Aminopeptidase P3, X-Pro aminopeptidase 3
Purification Purified by antigen affinity column.
Accession Q9NQH7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only.
Images

  • Immunofluorescence

    A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting XPNPEP3 (HV814014) at a concentration of 41.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of XPNPEP3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

  • Western blot

    Western blot analysis was performed using anti-XPNPEP3 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: 293T cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: PC3 cell lysate
    Lane 4: Mouse kidney lysate
    Lane 5: Mouse heart lysate

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Anti-XPNPEP3 Polyclonal Antibody

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