Anti-MYL9 Polyclonal Antibody (HB901014)

Name: Anti-MYL9 Polyclonal Antibody
Brand: abinScience
SKU: HB901014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB901014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MYL9 (Arg5-Leu163).

Target

Myosin regulatory light chain MRLC1, 20 kDa myosin light chain, Myosin regulatory light chain 2, smooth muscle isoform, MYRL2, MYL9, MLC-2C, LC20, Myosin regulatory light chain 9, MLC2, MRLC1, Myosin RLC, Myosin regulatory light polypeptide 9

Purification

Purified by antigen affinity column.

Accession

P24844

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Myosin regulatory light polypeptide 9 (MYL9) is a ~19 kDa protein. Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation.

1. Iwasaki, T. et al. (2001) Cell structure and function 26, 677-83. PMID: 11942626

2. Kumar, CC. et al. (1989) Biochemistry 28, 4027-35. PMID: 2526655

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MYL9 polyclonal antibody at 1μg/ml on various samples.
Lane 1: C2C12 cell lysate
Lane 2: Caco2 cell lysate
Lane 3: Daudi cell lysate
Lane 4: PC12 cell lysate
Lane 5: mouse Lung
Lane 6: Mouse colon lysate