Anti-LEF1 Polyclonal Antibody (HV355014)

Name: Anti-LEF1 Polyclonal Antibody
Brand: abinScience
SKU: HV355014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV355014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LEF1 (Glu45-His260).

Target

Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, LEF-1, TCF1-alpha, LEF1

Purification

Purified by antigen affinity column.

Accession

Q9UJU2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lymphoid enhancer-binding factor 1 (LEF1) is a ~44 kDa protein. Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.

1. Waterman, ML. et al. (1991) Genes & development 5, 656-69. PMID: 2010090

2. Brantjes, H. et al. (2001) Nucleic acids research 29, 1410-9. PMID: 11266540

3. Jesse, S. et al. (2010) International journal of cancer 126, 1109-20. PMID: 19653274

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LEF1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Lane 3: BxPC3 cell lysate
Lane 4: K562 cell lysate
Lane 5: Mouse thymus lysate

References

Datasheet

Anti-LEF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-LEF1 Polyclonal Antibody (HV355014)

Overview

Images

References

Datasheet

SDS

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