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Anti-AKR1B10 Polyclonal Antibody (HT001014)

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Overview
Catalog No.HT001014
Description
Anti-AKR1B10 Polyclonal Antibody (HT001014) is a rabbit polyclonal antibody detecting AKR1B10 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human AKR1B10 (Met1-Tyr316).
Target SI reductase, ARP, Small intestine reductase, ARL-1, Aldose reductase-related protein, Aldose reductase-like, AKR1B11, Aldo-keto reductase family 1 member B10, hARP, AKR1B10
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O60218
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Aldo-keto reductase family 1 member B10 (AKR1B10) is a ~36 kDa protein. Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose.

1. Crosas, B. et al. (2003) The Biochemical journal 373, 973-9. PMID: 12732097
2. Gallego, O. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20764-9. PMID: 18087047
3. Martin, HJ. et al. (2009) Chemico-biological interactions 178, 145-50. PMID: 19013440
4. Zhong, L. et al. (2009) Biochemical and biophysical research communications 387, 245-50. PMID: 19563777
5. Cao, D. et al. (1998) The Journal of biological chemistry 273, 11429-35. PMID: 9565553
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-AKR1B10 Polyclonal Antibody

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