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Anti-Mouse Polyclonal Antibody (MY077014)

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Overview
Catalog No.MY077014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse BMP1 (Glu615-Ser848).
Target Bone morphogenetic protein 1, BMP-1, 3.4.24.19, Mammalian tolloid protein, mTld, Procollagen C-proteinase, PCP, Bmp1
Purification Purified by antigen affinity column.
Accession P98063
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Bone morphogenetic protein 1 (BMP1) is a ~111 kDa protein. Metalloprotease that plays key roles in regulating the formation of the extracellular matrix (ECM) via processing of various precursor proteins into mature functional enzymes or structural proteins. Thereby participates in several developmental and physiological processes such as cartilage and bone formation, muscle growth and homeostasis, wound healing and tissue repair. Roles in ECM formation include cleavage of the C-terminal propeptides from procollagens such as procollagen I, II and III or the proteolytic activation of the enzyme lysyl oxidase LOX, necessary to formation of covalent cross-links in collagen and elastic fibers. Additional substrates include matricellular thrombospondin-1/THBS1 whose cleavage leads to cell adhesion disruption and TGF-beta activation.

1. Muir, AM. et al. (2014) Human molecular genetics 23, 3085-101. PMID: 24419319
2. Wang, J. et al. (2017) Journal of dental research 96, 578-585. PMID: 28068493
3. Suzuki, N. et al. (1996) Development (Cambridge, England) 122, 3587-95. PMID: 8951074
4. Maruhashi, T. et al. (2010) The Journal of biological chemistry 285, 13294-303. PMID: 20181949
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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