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Anti-Human HSD17B1 Polyclonal Antibody (HB193014)

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Overview
Catalog No.HB193014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human HSD17B1 (Thr4-Pro289).
Target EDH17B1, Short chain dehydrogenase/reductase family 28C member 1, EDHB17, Placental 17-beta-hydroxysteroid dehydrogenase, SDR28C1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, HSD17B1, 17-beta-HSD 1, E2DH, Estradiol 17-beta-dehydrogenase 1, EDH17B2, E17KSR, 17-beta-hydroxysteroid dehydrogenase type 1
Purification Purified by antigen affinity column.
Accession P14061
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

17-beta-hydroxysteroid dehydrogenase type 1 (HSD17B1) is a ~34 kDa protein. Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2). Also has 20-alpha-HSD activity. Uses preferentially NADH.

1. Puranen, T. et al. (1997) Molecular endocrinology (Baltimore, Md.) 11, 77-86. PMID: 8994190
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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