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Anti-MAP3K1 Polyclonal Antibody (HC601014)

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Overview
Catalog No.HC601014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MAP3K1 (Ile1307-Arg1509).
Target MAPK/ERK kinase kinase 1, Mitogen-activated protein kinase kinase kinase 1, MAP3K1, MEKK 1, MEKK, MEK kinase 1, MAPKKK1, MEKK1
Purification Purified by antigen affinity column.
Accession Q13233
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mitogen-activated protein kinase kinase kinase 1 (MAP3K1) is a ~164 kDa protein. Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

1. Xia, Y. et al. (1998) Genes & development 12, 3369-81. PMID: 9808624
2. Ye, B. et al. (2007) FEBS letters 581, 4403-10. PMID: 17761173
Note For research use only.
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Formula
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Anti-MAP3K1 Polyclonal Antibody

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