Anti-CTBS Polyclonal Antibody (HX869014)

Name: Anti-CTBS Polyclonal Antibody
Brand: abinScience
SKU: HX869014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HX869014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CTBS (Glu47-Arg385).

Target

CTB, Di-N-acetylchitobiase, CTBS

Purification

Purified by antigen affinity column.

Accession

Q01459

RRID

Anti-CTBS Polyclonal Antibody (abinScience Cat# HX869014, RRID:AB_3722901)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Di-N-acetylchitobiase (CTBS) is a ~43 kDa protein. Reducing-end exoglycosidase involved in glycan degradation. Hydrolyzes N,N'-diacetylchitobiose (GlnNAc2) and higher chitin-oligosaccharides (GlcNAc(n)) to monomeric GlcNAc acting on reducing-end residues. During N-linked glycoprotein degradation, it cleaves single GlcNAc molecules uniquely from the reducing end of N,N'-diacetylchitobiose units linking oligosaccharides to proteins, and therefore it functions after cleavage of the Asn-GlcNAc bond and asparagine removal by glycosylasparaginase.

1. Fisher, KJ. et al. (1992) The Journal of biological chemistry 267, 19607-16. PMID: 1527079

2. Park, C. et al. (2005) The Journal of biological chemistry 280, 37204-16. PMID: 16115860

3. Aronson, NN. et al. (2006) Bioscience, biotechnology, and biochemistry 70, 1537-41. PMID: 16794344

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CTBS polyclonal antibody at 1ug/mL on recombinant CTBS (Catalog No. HX869012).

References

Datasheet

Anti-CTBS Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CTBS Polyclonal Antibody (HX869014)

Overview

Images

References

Datasheet

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