Anti-CYGB Polyclonal Antibody (HV972014)

Name: Anti-CYGB Polyclonal Antibody
Brand: abinScience
SKU: HV972014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HV972014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYGB (Met1-Pro190).

Target

Histoglobin, CYGB, Stellate cell activation-associated protein, Cytoglobin, STAP, HGb

Purification

Purified by antigen affinity column.

Accession

Q8WWM9

RRID

Anti-CYGB Polyclonal Antibody (abinScience Cat# HV972014, RRID:AB_3722927)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytoglobin (CYGB) is a ~21 kDa protein. Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability. Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption. By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone. Under normoxic conditions functions as a nitric oxide dioxygenase (NOD) but under hypoxic conditions the globin may switch its function to that of a nitrite (NO2) reductase (NiR), generating nitric oxide. Could also have peroxidase and superoxide dismutase activities, detoxifying reactive oxygen species and protecting cells against oxidative stress.

1. Trent, JT. et al. (2002) The Journal of biological chemistry 277, 19538-45. PMID: 11893755

2. Asahina, K. et al. (2002) Biochimica et biophysica acta 1577, 471-5. PMID: 12359339

3. Sugimoto, H. et al. (2004) Journal of molecular biology 339, 873-85. PMID: 15165856

4. Halligan, KE. et al. (2009) The Journal of biological chemistry 284, 8539-47. PMID: 19147491

5. Gardner, AM. et al. (2010) The Journal of biological chemistry 285, 23850-7. PMID: 20511233

6. Liu, X. et al. (2017) Nature communications 8, 14807. PMID: 28393874

7. Amdahl, MB. et al. (2017) Biochemistry 56, 3993-4004. PMID: 28671819

8. Reeder, BJ. et al. (2018) Nitric oxide : biology and chemistry 72, 16-23. PMID: 29128400

9. Dat, NQ. et al. (2021) Hepatology (Baltimore, Md.) 73, 2527-2545. PMID: 33576020

10. Zweier, JL. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34930834

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CYGB polyclonal antibody at 1ug/mL on recombinant CYGB (Catalog No. HV972012).

References

Datasheet

Anti-CYGB Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CYGB Polyclonal Antibody (HV972014)

Overview

Images

References

Datasheet

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