Anti-GRIN2B Polyclonal Antibody (HC330014)

Name: Anti-GRIN2B Polyclonal Antibody
Brand: abinScience
SKU: HC330014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HC330014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GRIN2B (Tyr1133-Val1484).

Target

GluN2B, Glutamate receptor ionotropic, NMDA 2B, GRIN2B, NMDAR2B, NR2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl-D-aspartate receptor subunit 3, N-methyl D-aspartate receptor subtype 2B, hNR3, NR3

Purification

Purified by antigen affinity column.

Accession

Q13224

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glutamate receptor ionotropic, NMDA 2B (GRIN2B) is a ~166 kDa protein. Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents. Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators.

1. Lemke, JR. et al. (2014) Annals of neurology 75, 147-54. PMID: 24272827

2. Adams, DR. et al. (2014) Molecular genetics and metabolism 113, 161-70. PMID: 24863970

3. Hackos, DH. et al. (2016) Neuron 89, 983-99. PMID: 26875626

4. Volgraf, M. et al. (2016) Journal of medicinal chemistry 59, 2760-79. PMID: 26919761

5. Swanger, SA. et al. (2016) American journal of human genetics 99, 1261-1280. PMID: 27839871

6. Ogden, KK. et al. (2017) PLoS genetics 13, e1006536. PMID: 28095420

7. Chen, W. et al. (2017) Molecular pharmacology 91, 317-330. PMID: 28126851

8. Xu, Y. et al. (2024) Cellular and molecular life sciences : CMLS 81, 153. PMID: 38538865

9. Hess, SD. et al. (1996) The Journal of pharmacology and experimental therapeutics 278, 808-16. PMID: 8768735

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GRIN2B polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

References

Datasheet

Anti-GRIN2B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GRIN2B Polyclonal Antibody (HC330014)

Overview

Images

References

Datasheet

SDS

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