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Anti-ACSL1 Polyclonal Antibody (HW298014)

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Overview
Catalog No.HW298014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ACSL1 (Gly457-Val698).
Target FACL1, LACS2, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain-fatty-acid--CoA ligase 1, ACS1, LACS 1, Palmitoyl-CoA ligase 2, Phytanate--CoA ligase, FACL2, Arachidonate--CoA ligase, Long-chain fatty acid-CoA ligase 2, ACSL1, Acyl-CoA synthetase 1, Palmitoyl-CoA ligase 1, LACS1, LACS, LACS 2
Purification Purified by antigen affinity column.
Accession P33121
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Long-chain-fatty-acid--CoA ligase 1 (ACSL1) is a ~77 kDa protein. Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).

1. Golej, DL. et al. (2011) Journal of lipid research 52, 782-93. PMID: 21242590
2. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490
3. Ohkuni, A. et al. (2013) Biochemical and biophysical research communications 442, 195-201. PMID: 24269233
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ACSL1 Polyclonal Antibody

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