Anti-EGFR/ERBB1/HER1 Polyclonal Antibody (HF004014)

Name: Anti-EGFR/ERBB1/HER1 Polyclonal Antibody
Brand: abinScience
SKU: HF004014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HF004014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EGFR/ERBB1/HER1 (Arg670-Gly1022).

Target

HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB

Purification

Purified by antigen affinity column.

Accession

P00533

RRID

Anti-EGFR/ERBB1/HER1 Polyclonal Antibody (abinScience Cat# HF004014, RRID:AB_3723039)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix).

1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725

2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536

3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960

4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033

5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049

6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079

7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029

8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EGFR/ERBB1/HER1 polyclonal antibody at 1ug/mL on recombinant EGFR/ERBB1/HER1 (Catalog No. HF004032).

References

Datasheet

Anti-EGFR/ERBB1/HER1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EGFR/ERBB1/HER1 Polyclonal Antibody (HF004014)

Overview

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References

Datasheet

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