Anti-Human PRKCSH Polyclonal Antibody (HB154014)

Name: Anti-Human PRKCSH Polyclonal Antibody
Brand: abinScience
SKU: HB154014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet

Overview

Catalog No.

HB154014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PRKCSH (Asp401-Ala511).

Target

Glucosidase II subunit beta, Glucosidase 2 subunit beta, 80K-H protein, G19P1, PKCSH, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain

Purification

Purified by antigen affinity column.

Accession

P14314

RRID

Anti-Human PRKCSH Polyclonal Antibody (abinScience Cat# HB154014, RRID:AB_3723174)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glucosidase 2 subunit beta (PRKCSH) is a ~59 kDa protein. Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.

1. Pelletier, MF. et al. (2000) Glycobiology 10, 815-27. PMID: 10929008

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PRKCSH polyclonal antibody at 1µg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A549 cell lysate
Lane 5: SKOV3 cell lysate
Lane 6: Daudi cell lysate
Lane 7: K562 cell lysate
Lane 8: U87 cell lysate
Lane 9: Mouse liver lysate
Lane 10: Rat liver lysate

References

Datasheet

Anti-Human PRKCSH Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PRKCSH Polyclonal Antibody (HB154014)

Overview

Images

References

Datasheet

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