Anti-Human E2F4 Polyclonal Antibody (HS944014)

Name: Anti-Human E2F4 Polyclonal Antibody
Brand: abinScience
SKU: HS944014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HS944014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human E2F4 (Ser16-Lys82).

Target

E2F4, Transcription factor E2F4, E2F-4

Purification

Purified by antigen affinity column.

Accession

Q16254

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor E2F4 is a ~43 kDa protein. Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human E2F4 polyclonal antibody at 1ug/mL on recombinant Human E2F4 (Catalog No. HS944012).

References

Datasheet

Anti-Human E2F4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human E2F4 Polyclonal Antibody (HS944014)

Overview

Images

References

Datasheet

SDS

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