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Anti-EARS2 Polyclonal Antibody (HP762014)

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Overview
Catalog No.HP762014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human EARS2 (Gly247-Gln496).
Target KIAA1970, Glutamyl-tRNA synthetase, EARS2, Probable glutamate--tRNA ligase, mitochondrial, GluRS
Purification Purified by antigen affinity column.
Accession Q5JPH6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nondiscriminating glutamyl-tRNA synthetase EARS2, mitochondrial is a ~58 kDa protein. Non-discriminating glutamyl-tRNA synthetase that catalyzes aminoacylation of both mitochondrial tRNA(Glu) and tRNA(Gln) and participates in RNA aminoacylation for mitochondrial protein translation. Attachs glutamate to tRNA(Glu) or tRNA(Gln) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) or tRNA(Gln). In vitro, cytoplasmic tRNA(Gln) is slightly glutamylated, but with low activity.

1. Nagao, A. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 16209-14. PMID: 19805282
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-EARS2 Polyclonal Antibody

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