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Anti-Human CTPS2 Polyclonal Antibody (HP851014)

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Overview
Catalog No.HP851014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CTPS2 (Met1-Lys297).
Target CTPS2, CTP synthetase 2, UTP--ammonia ligase 2, CTP synthase 2
Purification Purified by antigen affinity column.
Accession Q9NRF8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

CTP synthase 2 (CTPS2) is a ~65 kDa protein. CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. Constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

1. van Kuilenburg, AB. et al. (2000) Biochimica et biophysica acta 1492, 548-52. PMID: 10899599
2. Han, GS. et al. (2005) The Journal of biological chemistry 280, 38328-36. PMID: 16179339
3. Lynch, EM. et al. (2020) Nature structural & molecular biology 27, 42-48. PMID: 31873303
4. Lynch, EM. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34583994
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human CTPS2 Polyclonal Antibody

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