Anti-Human MUL1 Polyclonal Antibody (HP112014)

Name: Anti-Human MUL1 Polyclonal Antibody
Brand: abinScience
SKU: HP112014
Price: 96 USD
Availability: InStock

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Spec:

20ug
50ug
100ug

Number:

Overview
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References
Datasheet

Overview

Catalog No.

HP112014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MUL1 (Lys44-Gln224).

Target

Mitochondrial-anchored protein ligase, E3 ubiquitin-protein ligase MUL1, Protein Hades, C1orf166, RNF218, Mitochondrial ubiquitin ligase activator of NFKB 1, Growth inhibition and death E3 ligase, MUL1, RING finger protein 218, MAPL, RING-type E3 ubiquitin transferase NFKB 1, Putative NF-kappa-B-activating protein 266, GIDE, MULAN, E3 SUMO-protein ligase MUL1

Purification

Purified by antigen affinity column.

Accession

Q969V5

RRID

Anti-Human MUL1 Polyclonal Antibody (abinScience Cat# HP112014, RRID:AB_3723436)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitochondrial ubiquitin ligase activator of NFKB 1 (MUL1) is a ~39 kDa protein. Exhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteasomal degradation. Mediates polyubiquitination of cytoplasmic TP53 at 'Lys-24' which targets TP53 for proteasomal degradation, thus reducing TP53 levels in the cytoplasm and mitochondrion. Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations.

1. Zhang, B. et al. (2008) Cell research 18, 900-10. PMID: 18591963

2. Braschi, E. et al. (2009) EMBO reports 10, 748-54. PMID: 19407830

3. Bae, S. et al. (2012) Cell research 22, 873-85. PMID: 22410793

4. Jung, JH. et al. (2011) Cell death and differentiation 18, 1865-75. PMID: 21597459

5. Neuspiel, M. et al. (2008) Current biology : CB 18, 102-8. PMID: 18207745

7. Yun, J. et al. (2014) eLife 3, e01958. PMID: 24898855

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse skeletal muscle lysate
Lane 2: Rat skeletal muscle lysate

References

Datasheet

Anti-Human MUL1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MUL1 Polyclonal Antibody (HP112014)

Overview

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References

Datasheet

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