Anti-Mouse CCN3 Polyclonal Antibody (MW350014)

Name: Anti-Mouse CCN3 Polyclonal Antibody
Brand: abinScience
SKU: MW350014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MW350014

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CCN3 (Ser50-Arg137).

Target

CCN family member 3 (Cellular communication network factor 3) (Nephroblastoma-overexpressed gene protein homolog) (Protein NOV homolog) (NovH)

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q64299, Q9QZQ5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CCN family member 3 (CCN3) is a ~38 kDa protein. Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling.

1. Matsushita, Y. et al. (2013) The Journal of biological chemistry 288, 19973-85. PMID: 23653360

2. Paradis, R. et al. (2013) PloS one 8, e64957. PMID: 23705021

3. Liu, J. et al. (2014) PloS one 9, e94912. PMID: 24722330

4. Babey, ME. et al. (2024) Nature 632, 357-365. PMID: 38987585

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Mouse CCN3 polyclonal antibody at 1ug/mL on recombinant Mouse CCN3 (Catalog No. MW350012).

References

Datasheet

Anti-Mouse CCN3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CCN3 Polyclonal Antibody (MW350014)

Overview

Images

References

Datasheet

SDS

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