Anti-Mouse IL4I1 Polyclonal Antibody (MP675014)

Name: Anti-Mouse IL4I1 Polyclonal Antibody
Brand: abinScience
SKU: MP675014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

MP675014

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse IL4I1 (Asp40-Gly507).

Target

L-amino-acid oxidase (LAAO) (LAO) (EC 1.4.3.2) (EC 1.4.3.25) (Interleukin-4-induced protein 1) (IL4-induced protein 1) (mIL4I1) (Protein Fig-1) (mFIG1)

Purification

Purified by antigen affinity column.

Accession

O09046

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

L-amino-acid oxidase (IL4I1) is a ~70 kDa protein. Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer.

1. Sadik, A. et al. (2020) Cell 182, 1252-1270.e34. PMID: 32818467

2. Mason, JM. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 4561-7. PMID: 15383589

3. Lasoudris, F. et al. (2011) European journal of immunology 41, 1629-38. PMID: 21469114

4. Bod, L. et al. (2017) Oncoimmunology 6, e1278331. PMID: 28405502

5. Cousin, C. et al. (2015) European journal of immunology 45, 1772-82. PMID: 25778793

6. Bod, L. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1027-1038. PMID: 29288206

7. Yue, Y. et al. (2015) PloS one 10, e0142979. PMID: 26599209

Note

For research use only.

Images

References

Datasheet

Anti-Mouse IL4I1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Mouse IL4I1 Polyclonal Antibody (MP675014)

Overview

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References

Datasheet

SDS

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