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Anti-Human HNRNPL Polyclonal Antibody (HB914014)

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Overview
Catalog No.HB914014
Description
Anti-Human HNRNPL Polyclonal Antibody (HB914014) is a rabbit polyclonal antibody detecting HNRNPL in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HNRNPL (Ser381-Ser589).
Target hnRNP L, HNRPL, Heterogeneous nuclear ribonucleoprotein L, HNRNPL
Purification Purified by antigen affinity column.
Accession P14866
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Heterogeneous nuclear ribonucleoprotein L (HNRNPL) is a ~64 kDa protein. Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPK and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation.

1. Kuninger, DT. et al. (2002) Nucleic acids research 30, 823-9. PMID: 11809897
2. Liu, G. et al. (2012) The Journal of biological chemistry 287, 22709-16. PMID: 22570490
3. Shankarling, G. et al. (2014) Molecular and cellular biology 34, 71-83. PMID: 24164894
4. Loh, TJ. et al. (2015) Biochimica et biophysica acta 1849, 743-50. PMID: 25623890
5. Blatter, M. et al. (2015) Journal of molecular biology 427, 3001-22. PMID: 26051023
6. Piñol-Roma, S. et al. (1989) The Journal of cell biology 109, 2575-87. PMID: 2687284
7. Hollensen, AK. et al. (2020) eLife 9. PMID: 33174841
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human HNRNPL Polyclonal Antibody

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