Anti-Human ATP5PO Polyclonal Antibody (HW696014)

Name: Anti-Human ATP5PO Polyclonal Antibody
Brand: abinScience
SKU: HW696014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW696014

Description

Anti-Human ATP5PO Polyclonal Antibody (HW696014) is a rabbit polyclonal antibody detecting ATP5PO in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP5PO (Gly36-Val213).

Target

ATP synthase peripheral stalk subunit OSCP, ATP5O, Oligomycin sensitivity conferral protein, ATP5PO, OSCP, ATPO, ATP synthase subunit O, mitochondrial

Purification

Purified by antigen affinity column.

Accession

P48047

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP synthase peripheral stalk subunit OSCP, mitochondrial (ATP5PO) is a ~23 kDa protein. Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ATP5PO polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: SKOV3 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Hela cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse heart lysate
Lane 10: Mouse liver lysate
Lane11: Rat brain lysate
Lane12: Rat heart lysate
Lane13: Rat liver lysate

References

Datasheet

Anti-Human ATP5PO Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP5PO Polyclonal Antibody (HW696014)

Overview

Images

References

Datasheet

SDS

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