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Anti-Human TPM4 Polyclonal Antibody (HX004014)

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Anti-Human TPM4 Polyclonal Antibody
Anti-Human TPM4 Polyclonal Antibody
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Overview
Catalog No.HX004014
Description
Anti-Human TPM4 Polyclonal Antibody (HX004014) is a rabbit polyclonal antibody detecting TPM4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TPM4 (Met1-Ile248).
Target Tropomyosin alpha-4 chain, Tropomyosin-4, TM30p1, TPM4
Purification Purified by antigen affinity column.
Accession P67936
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tropomyosin alpha-4 chain (TPM4) is a ~28 kDa protein. Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.

1. Crabos, M. et al. (1991) European journal of clinical investigation 21, 472-8. PMID: 1836432
Note For research use only.
Images
  • Anti-Human TPM4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TPM4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: A431 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: Jurkat cell lysate
    Lane 7: U87 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human TPM4 Polyclonal Antibody

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