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Anti-Human ATP13A1 Polyclonal Antibody (HA160014)

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Anti-Human ATP13A1 Polyclonal Antibody
Anti-Human ATP13A1 Polyclonal Antibody
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Overview
Catalog No.HA160014
Description
Anti-Human ATP13A1 Polyclonal Antibody (HA160014) is a rabbit polyclonal antibody detecting ATP13A1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ATP13A1 (Ser542-Ser722).
Target Endoplasmic reticulum P5A-ATPase, KIAA1825, ATP13A1, Endoplasmic reticulum transmembrane helix translocase, ATP13A
Purification Purified by antigen affinity column.
Accession Q9HD20
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Endoplasmic reticulum transmembrane helix translocase (ATP13A1) is a ~132 kDa protein. Endoplasmic reticulum translocase required to remove mitochondrial transmembrane proteins mistargeted to the endoplasmic reticulum. Acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane. Specifically binds mitochondrial tail-anchored transmembrane proteins: has an atypically large substrate-binding pocket that recognizes and binds moderately hydrophobic transmembranes with short hydrophilic lumenal domains.

1. McKenna, MJ. et al. (2020) Science (New York, N.Y.) 369. PMID: 32973005
2. Guna, A. et al. (2022) Science (New York, N.Y.) 378, 317-322. PMID: 36264797
Note For research use only.
Images
  • Anti-Human ATP13A1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ATP13A1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse brain lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ATP13A1 Polyclonal Antibody

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