Anti-Human AKAP8 Polyclonal Antibody (HT110014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HT110014

Description

Anti-Human AKAP8 Polyclonal Antibody (HT110014) is a rabbit polyclonal antibody detecting AKAP8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AKAP8 (Met1-Glu572).

Target

AKAP8, A-kinase anchor protein 8, AKAP95, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95

Purification

Purified by antigen affinity column.

Accession

O43823

RRID

Anti-Human AKAP8 Polyclonal Antibody (abinScience Cat# HT110014, RRID:AB_3724138)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

A-kinase anchor protein 8 (AKAP8) is a ~76 kDa protein. Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis.

1. Eide, T. et al. (1998) Experimental cell research 238, 305-16. PMID: 9473338

2. Collas, P. et al. (1999) The Journal of cell biology 147, 1167-80. PMID: 10601332

3. Steen, RL. et al. (2000) The Journal of cell biology 149, 531-6. PMID: 10791967

4. Eide, T. et al. (2002) EMBO reports 3, 426-32. PMID: 11964380

5. Arsenijevic, T. et al. (2004) The Biochemical journal 378, 673-9. PMID: 14641107

6. Li, Y. et al. (2006) Genes & development 20, 2566-79. PMID: 16980585

7. Gao, X. et al. (2012) Molecular biology of the cell 23, 503-15. PMID: 22130794

8. Eide, T. et al. (2003) The Journal of biological chemistry 278, 26750-6. PMID: 12740381

9. Jiang, H. et al. (2013) Nature structural & molecular biology 20, 1156-63. PMID: 23995757

10. Kamada, S. et al. (2005) Molecular and cellular biology 25, 9469-77. PMID: 16227597

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human AKAP8 polyclonal antibody at 1ug/mL on recombinant Human AKAP8 (Catalog No. HT110012).

References

Datasheet

Anti-Human AKAP8 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human AKAP8 Polyclonal Antibody (HT110014)

Overview

Images

References

Datasheet

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