Anti-JUN Polyclonal Antibody (HY261014)

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50ug
100ug

Number:

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Datasheet

Overview

Catalog No.

HY261014

Description

Anti-JUN Polyclonal Antibody (HY261014) is a rabbit polyclonal antibody detecting JUN in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human JUN (Met1-Phe331).

Target

Proto-oncogene c-Jun, JUN, V-jun avian sarcoma virus 17 oncogene homolog, Transcription factor AP-1, AP1, Activator protein 1, p39

Purification

Purified by antigen affinity column.

Accession

P05412

RRID

Anti-JUN Polyclonal Antibody (abinScience Cat# HY261014, RRID:AB_3724150)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor Jun is a ~35 kDa protein. Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity. Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

1. Qing, J. et al. (2000) The Journal of biological chemistry 275, 38802-12. PMID: 10995748

2. Ji, Z. et al. (2012) Molecular and cellular biology 32, 385-98. PMID: 22083952

3. Baumann, S. et al. (2003) Oncogene 22, 1333-9. PMID: 12618758

4. Lan, HC. et al. (2007) Molecular and cellular biology 27, 2027-36. PMID: 17210646

5. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-JUN polyclonal antibody at 1ug/mL on recombinant JUN (Catalog No. HY261022).

References

Datasheet

Anti-JUN Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-JUN Polyclonal Antibody (HY261014)

Overview

Images

References

Datasheet

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