Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014)

Name: Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody
Brand: abinScience
SKU: VK686014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK686014

Description

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014) is a rabbit polyclonal antibody detecting EBNA1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant EBV/HHV4 EBNA1/BKRF1 (His468-Pro607).

Target

Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1

Purification

Purified by antigen affinity column.

Accession

P03211

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Epstein-Barr nuclear antigen 1 (EBNA1/BKRF1) is a ~56 kDa protein. Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency. EBNA1 binding to DS recruits the host origin recognition complex (ORC). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes. Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes.

1. Sears, J. et al. (2004) Journal of virology 78, 11487-505. PMID: 15479791

2. Rawlins, DR. et al. (1985) Cell 42, 859-68. PMID: 2996781

3. Hodin, TL. et al. (2013) Journal of virology 87, 13020-8. PMID: 24067969

4. Malecka, KA. et al. (2019) Journal of virology 93. PMID: 31142669

5. Summers, H. et al. (1996) Journal of virology 70, 1228-31. PMID: 8551585

6. Ritzi, M. et al. (2003) Journal of cell science 116, 3971-84. PMID: 12953058

7. Hung, SC. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1865-70. PMID: 11172042

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EBV/HHV4 EBNA1/BKRF1 polyclonal antibody at 1ug/mL on recombinant EBV/HHV4 EBNA1/BKRF1 (Catalog No. VK686012).
Western blot

Western blot analysis was performed using anti-EBNA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV4 EBNA1/BKRF1 (Catalog No: VK686012)