Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody (HX034014)

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100ug

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Datasheet

Overview

Catalog No.

HX034014

Description

Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody (HX034014) is a rabbit polyclonal antibody detecting Beta-actin in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human β-actin (Met1-Phe375).

Target

Beta-actin, ACTB, Actin, cytoplasmic 1

Purification

Purified by antigen affinity column.

Accession

P60709

RRID

Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody (abinScience Cat# HX034014, RRID:AB_3724182)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Actin, cytoplasmic 1 (ACTB/β-actin) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built.

1. Hundt, N. et al. (2014) The FEBS journal 281, 5279-91. PMID: 25255767

2. Drazic, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4399-4404. PMID: 29581253

3. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

4. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809

5. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ACTB/β-actin/Beta Actin polyclonal antibody at 1ug/mL on recombinant ACTB/β-actin/Beta Actin (Catalog No. HX058012).

References

Datasheet