Anti-Human NKRF Polyclonal Antibody (HT276014)

Name: Anti-Human NKRF Polyclonal Antibody
Brand: abinScience
SKU: HT276014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HT276014

Description

Anti-Human NKRF Polyclonal Antibody (HT276014) is a rabbit polyclonal antibody detecting NKRF in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NKRF (Phe162-Thr415).

Target

NF-kappa-B-repressing factor, NFkB-repressing factor, NRF, Protein ITBA4, NKRF, ITBA4, NRF

Purification

Purified by antigen affinity column.

Accession

O15226

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NF-kappa-B-repressing factor (NKRF) is a ~77 kDa protein. Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes.

1. Feng, X. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14212-7. PMID: 12381793

2. Nourbakhsh, M. et al. (1999) The EMBO journal 18, 6415-25. PMID: 10562553

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NKRF polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: K562 cell lysate
Lane 7: A549 cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat spleen lysate

References

Datasheet

Anti-Human NKRF Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NKRF Polyclonal Antibody (HT276014)

Overview

Images

References

Datasheet

SDS

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