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Anti-Human PLRG1 Polyclonal Antibody (HT092014)

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Anti-Human PLRG1 Polyclonal Antibody
Anti-Human PLRG1 Polyclonal Antibody
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Overview
Catalog No.HT092014
Description
Anti-Human PLRG1 Polyclonal Antibody (HT092014) is a rabbit polyclonal antibody detecting PLRG1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PLRG1 (Trp194-Phe514).
Target Pleiotropic regulator 1, PLRG1
Purification Purified by antigen affinity column.
Accession O43660
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Pleiotropic regulator 1 (PLRG1) is a ~57 kDa protein. Involved in pre-mRNA splicing as component of the spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).

1. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346
2. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770
3. Ajuh, P. et al. (2000) The EMBO journal 19, 6569-81. PMID: 11101529
4. Ajuh, P. et al. (2001) The Journal of biological chemistry 276, 42370-81. PMID: 11544257
Note For research use only.
Images
  • Anti-Human PLRG1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PLRG1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Raji cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PLRG1 Polyclonal Antibody

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