Anti-Human MAFK Polyclonal Antibody (HT106014)

Name: Anti-Human MAFK Polyclonal Antibody
Brand: abinScience
SKU: HT106014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT106014

Description

Anti-Human MAFK Polyclonal Antibody (HT106014) is a rabbit polyclonal antibody detecting MAFK in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAFK (Met1-Ser156).

Target

Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK

Purification

Purified by antigen affinity column.

Accession

O60675

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor MafK is a ~17 kDa protein. Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

1. Toki, T. et al. (1997) Oncogene 14, 1901-10. PMID: 9150357

2. Johnsen, O. et al. (1996) Nucleic acids research 24, 4289-97. PMID: 8932385

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAFK polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate
Lane 3: Mouse lung lysate

References

Datasheet

Anti-Human MAFK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MAFK Polyclonal Antibody (HT106014)

Overview

Images

References

Datasheet

SDS

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