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Anti-Human FAM20B Polyclonal Antibody (HF805014)

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Anti-Human FAM20B Polyclonal Antibody
Anti-Human FAM20B Polyclonal Antibody
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Overview
Catalog No.HF805014
Description
Anti-Human FAM20B Polyclonal Antibody (HF805014) is a rabbit polyclonal antibody detecting FAM20B in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human FAM20B (Gln61-Leu409).
Target Glycosaminoglycan xylosylkinase, 2.7.1.-, Xylose kinase, FAM20B, KIAA0475
Purification Purified by antigen affinity column.
Accession O75063
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycosaminoglycan xylosylkinase (FAM20B) is a ~46 kDa protein. Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized.

Note For research use only.
Images
  • Anti-Human FAM20B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FAM20B polyclonal antibody at FAM20B on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse cerebellum lysate
    Lane 3: Rat brain lysate
    Lane 4: Rat cerebellum lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human FAM20B Polyclonal Antibody

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