Anti-Human E2F6 Polyclonal Antibody (HF932014)

Name: Anti-Human E2F6 Polyclonal Antibody
Brand: abinScience
SKU: HF932014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF932014

Description

Anti-Human E2F6 Polyclonal Antibody (HF932014) is a rabbit polyclonal antibody detecting E2F6 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human E2F6 (Pro62-Glu237).

Target

Transcription factor E2F6, E2F-6, E2F6

Purification

Purified by antigen affinity column.

Accession

O75461

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor E2F6 is a ~31 kDa protein. Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression.

1. Trimarchi, JM. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 2850-5. PMID: 9501179

2. Gaubatz, S. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 9190-5. PMID: 9689056

3. Cartwright, P. et al. (1998) Oncogene 17, 611-23. PMID: 9704927

Note

For research use only.

Images

References

Datasheet

Anti-Human E2F6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human E2F6 Polyclonal Antibody (HF932014)

Overview

Images

References

Datasheet

SDS

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