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Anti-Human H2AB1 Polyclonal Antibody (HY202014)

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Overview
Catalog No.HY202014
Description
Anti-Human H2AB1 Polyclonal Antibody (HY202014) is a rabbit polyclonal antibody detecting H2AB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human H2AB1 (Met1-Asp115).
Target Histone H2A-Bbd type 1, H2A Barr body-deficient, H2A.B, H2A.Bbd, H2AB1, H2AFB1
Purification Purified by antigen affinity column.
Accession P0C5Y9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Histone H2A-Bbd type 1 (H2AB1) is a ~12 kDa protein. Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase.

1. Tolstorukov, MY. et al. (2012) Molecular cell 47, 596-607. PMID: 22795134
2. Bao, Y. et al. (2004) The EMBO journal 23, 3314-24. PMID: 15257289
3. Okuwaki, M. et al. (2005) Molecular and cellular biology 25, 10639-51. PMID: 16287874
4. Doyen, CM. et al. (2006) The EMBO journal 25, 4234-44. PMID: 16957777
5. Menoni, H. et al. (2007) Molecular and cellular biology 27, 5949-56. PMID: 17591702
6. Eirín-López, JM. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 316-26. PMID: 17726088
7. González-Romero, R. et al. (2008) Gene 413, 1-7. PMID: 18329190
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human H2AB1 Polyclonal Antibody

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