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Anti-Human UBAP1 Polyclonal Antibody (HA074014)

Anti-Human UBAP1 Polyclonal Antibody
Anti-Human UBAP1 Polyclonal Antibody
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Overview
Catalog No.HA074014
Description
Anti-Human UBAP1 Polyclonal Antibody (HA074014) is a rabbit polyclonal antibody detecting UBAP1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human UBAP1 (Ala381-Ser502).
Target Ubiquitin-associated protein 1, UBAP-1, Nasopharyngeal carcinoma-associated gene 20 protein, UBAP1, NAG20
Purification Purified by antigen affinity column.
Accession Q9NZ09
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-associated protein 1 (UBAP1) is a ~55 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2.

1. Stefani, F. et al. (2011) Current biology : CB 21, 1245-50. PMID: 21757351
2. Agromayor, M. et al. (2012) Structure (London, England : 1993) 20, 414-28. PMID: 22405001
3. Lin, X. et al. (2019) Brain : a journal of neurology 142, 2238-2252. PMID: 31203368
4. Wunderley, L. et al. (2014) Journal of cell science 127, 663-72. PMID: 24284069
Note For research use only.
Images
  • Anti-Human UBAP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-UBAP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: A431 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: Mosue brain lysate
    Lane 4: Mouse testis lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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