Anti-Human Lambda light chain Polyclonal Antibody (HA011014)

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Overview
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Datasheet

Overview

Catalog No.

HA011014

Description

Anti-Human Lambda light chain Polyclonal Antibody (HA011014) is a rabbit polyclonal antibody detecting Ig lambda-1 chain C region in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IGLC1 (Gly1-Thr95).

Target

Immunoglobulin lambda constant 1, Ig lambda chain C region MGC, Ig lambda-1 chain C region, IGLC1

Purification

Purified by antigen affinity column.

Accession

P0CG04

RRID

Anti-Human Lambda light chain Polyclonal Antibody (abinScience Cat# HA011014, RRID:AB_3724690)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Immunoglobulin lambda constant 1 (IGLC1) is a ~11 kDa protein. Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268

2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414

3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IGLC1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse serum lysate
Lane 2: Rat thymus lysate

References

Datasheet

Anti-Human Lambda light chain Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Lambda light chain Polyclonal Antibody (HA011014)

Overview

Images

References

Datasheet

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