Anti-Mouse GPX1 Polyclonal Antibody (MY365014)

Name: Anti-Mouse GPX1 Polyclonal Antibody
Brand: abinScience
SKU: MY365014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY365014

Description

Anti-Mouse GPX1 Polyclonal Antibody (MY365014) is a rabbit polyclonal antibody detecting Gpx1 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse GPX1 (Gly48-Ser201).

Target

Glutathione peroxidase 1, GPx-1, GSHPx-1, 1.11.1.9, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, 1.11.1.12, Selenium-dependent glutathione peroxidase 1, Gpx1

Purification

Purified by antigen affinity column.

Accession

P11352

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glutathione peroxidase 1 (GPX1) is a ~22 kDa protein. Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis. Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides. In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway.

1. Esposito, LA. et al. (2000) Free radical biology & medicine 28, 754-66. PMID: 10754271

2. Wang, SK. et al. (2011) Free radical biology & medicine 51, 197-204. PMID: 21420488

3. Schwarz, M. et al. (2023) Redox biology 59, 102593. PMID: 36608588

4. Esworthy, RS. et al. (1997) Archives of biochemistry and biophysics 340, 59-63. PMID: 9126277

5. Ho, YS. et al. (1997) The Journal of biological chemistry 272, 16644-51. PMID: 9195979

6. de Haan, JB. et al. (1998) The Journal of biological chemistry 273, 22528-36. PMID: 9712879

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GPX1 polyclonal antibody at GPX1 on various samples.
Lane 1: Mouse liver lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse spleen lysate
Lane 4: Mouse lung lysate
Lane 5: Rat liver lysate

References

Datasheet

Anti-Mouse GPX1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse GPX1 Polyclonal Antibody (MY365014)

Overview

Images

References

Datasheet

SDS

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