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Anti-BCCV N/Nucleoprotein Polyclonal Antibody (VK484014)

Anti-BCCV N/Nucleoprotein Polyclonal Antibody
Anti-BCCV N/Nucleoprotein Polyclonal Antibody
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Overview
Catalog No.VK484014
Description
Anti-BCCV N/Nucleoprotein Polyclonal Antibody (VK484014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityBlack Creek Canal orthohantavirus (BCCV) (Black Creek Canal virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant BCCV N/Nucleoprotein (Met1-Ile428).
Target Nucleoprotein, 3.1.-.-, Nucleocapsid protein, Protein N, N
Purification Purified by antigen affinity column.
Accession I7GVL4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein is a ~47 kDa protein. Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable). The nucleocapsid has a left-handed helical structure. As a trimer, specifically binds and acts as a chaperone to unwind the panhandle structure formed by the viral RNA (vRNA) termini. Involved in the transcription and replication initiation of vRNA by mediating primer annealing.

Note For research use only.
Images
  • Anti-BCCV N/Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-BCCV N/Nucleoprotein polyclonal antibody at 1ug/mL on recombinant BCCV N/Nucleoprotein (Catalog No. VK484012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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