Anti-TJP1 Polyclonal Antibody (HC432014)

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Datasheet

Overview

Catalog No.

HC432014

Description

Anti-TJP1 Polyclonal Antibody (HC432014) is a rabbit polyclonal antibody detecting TJP1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TJP1 (Met1-Val190).

Target

TJP1, ZO1, Zonula occludens protein 1, Zona occludens protein 1, Tight junction protein ZO-1, Tight junction protein 1

Purification

Purified by antigen affinity column.

Accession

Q07157

RRID

Anti-TJP1 Polyclonal Antibody (abinScience Cat# HC432014, RRID:AB_3724917)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tight junction protein 1 (TJP1) is a ~195 kDa protein. TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

1. Furuse, M. et al. (1994) The Journal of cell biology 127, 1617-26. PMID: 7798316

2. Fanning, AS. et al. (1998) The Journal of biological chemistry 273, 29745-53. PMID: 9792688

3. Huo, L. et al. (2011) The EMBO journal 30, 665-78. PMID: 21240187

4. Kremerskothen, J. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 505-14. PMID: 20930113

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TJP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

References

Datasheet

Anti-TJP1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TJP1 Polyclonal Antibody (HC432014)

Overview

Images

References

Datasheet

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