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Anti-Human PCMT1 Polyclonal Antibody (HB868014)

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Anti-Human PCMT1 Polyclonal Antibody
Anti-Human PCMT1 Polyclonal Antibody
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Overview
Catalog No.HB868014
Description
Anti-Human PCMT1 Polyclonal Antibody (HB868014) is a rabbit polyclonal antibody detecting PCMT1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PCMT1 (Met1-Lys227).
Target PIMT, L-isoaspartyl protein carboxyl methyltransferase, PCMT1, Protein-L-isoaspartate(D-aspartate) O-methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase
Purification Purified by antigen affinity column.
Accession P22061
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT1) is a ~24 kDa protein. Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.

1. Gilbert, JM. et al. (1988) Biochemistry 27, 5227-33. PMID: 3167043
2. Murray, ED. et al. (1984) The Journal of biological chemistry 259, 10722-32. PMID: 6469980
Note For research use only.
Images
  • Anti-Human PCMT1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PCMT1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: 293T cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: Mouse testis lysate
    Lane 6: Mouse heart lysate
    Lane 7: Rat testis lysate
    Lane 8: Rat heart lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PCMT1 Polyclonal Antibody

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